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Merck KGaA
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CU CPT 22 is a selective TLR1/2 inhibitor (IC50 = 0.58 μM).
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Image Search Results
Journal: GeroScience
Article Title: Contribution of Porphyromonas gingivalis lipopolysaccharide to experimental periodontitis in relation to aging
doi: 10.1007/s11357-020-00258-1
Figure Lengend Snippet: Age-dependent expressions of Pg-LPS receptors, TLR2, and TLR4, on the surface of CD11b+CSF1r-eGFP+ OCPs. Osteoclast precursors (OCPs) were FACS sorted from the spleen of young (2 months old) and aged (24 months old) CSF1r-eGFP-KI mice and then were stimulated with macrophage colony-stimulating factor (M-CSF) (30 ng/ml) and RANKL (5 ng/ml). After 24 h of stimulation, the expression of TLR2 (a and b) and TLR4 (c and d) was evaluated by multicolor flow cytometry. ***p < 0.001
Article Snippet: FACS-sorted CD11b+CSF1r-eGFP+ OCPs were seeded in 96-well plates at a density of 1 × 10 4 cells/well in α-MEM medium (Life Technologies) containing 10% FBS (Atlanta Biologicals) and 30 ng/ml of M-CSF and 5 ng/ml of RANKL (BioLegend) recombinant proteins in the presence or absence of various concentrations of ultrapure Pg -LPS (1, or 10 ng/ml; InvivoGen),
Techniques: Expressing, Flow Cytometry
Journal: GeroScience
Article Title: Contribution of Porphyromonas gingivalis lipopolysaccharide to experimental periodontitis in relation to aging
doi: 10.1007/s11357-020-00258-1
Figure Lengend Snippet: The effects of Pg-LPS and TLR’s antagonists on RANKL-induced osteoclastogenesis in relation to aging. Microscopic evaluation (a) of the TRAP staining and quantification of the number of TRAP+ multinucleated cells (b) in the RANKL-stimulated young and aged OCPs exposed to Pg-LPS in vitro. Effects of TLR4 (CAS 243984-11-4) and TLR2 antagonists (CU-CPT22) on young (c) and aged (d) RANKL-stimulated OCPs exposed to Pg-LPS. OCPs were FACS sorted from the spleen of young and aged mice and then were stimulated with RANKL in the presence or absence of various concentrations of Pg-LPS as well as TLR2 and TLR4 antagonists. The cells were evaluated at day 6 after stimulation. **p < 0.01, ***p < 0.001
Article Snippet: FACS-sorted CD11b+CSF1r-eGFP+ OCPs were seeded in 96-well plates at a density of 1 × 10 4 cells/well in α-MEM medium (Life Technologies) containing 10% FBS (Atlanta Biologicals) and 30 ng/ml of M-CSF and 5 ng/ml of RANKL (BioLegend) recombinant proteins in the presence or absence of various concentrations of ultrapure Pg -LPS (1, or 10 ng/ml; InvivoGen),
Techniques: Staining, In Vitro